Involvement of DNA polymerase alpha in host cell reactivation of UV-irradiated herpes simplex virus

J Virol. 1984 Feb;49(2):598-600. doi: 10.1128/JVI.49.2.598-600.1984.


Aphidicolin is a potent inhibitor of both host cell DNA polymerase alpha and herpes simplex virus (HSV)-induced DNA polymerase but has no effect on DNA polymerases beta and gamma of host cells. By using an aphidicolin-resistant mutant (Aphr) of HSV, a possible involvement of DNA polymerase alpha in host cell reactivation of UV-damaged HSV was studied. Plaque formation by UV-irradiated Aphr was markedly inhibited by 1 microgram of aphidicolin per ml, which did not affect the plating efficiency of nonirradiated Aphr. Aphidicolin added before 12 h postinfection inhibited plaque formation by irradiated Aphr, which became aphidicolin insensitive after 36 h postinfection. The results strongly suggest that host cell DNA polymerase alpha is involved in the repair of UV-irradiated HSV DNA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aphidicolin
  • Cell Transformation, Viral / radiation effects*
  • DNA Polymerase II / antagonists & inhibitors*
  • Diterpenes / pharmacology*
  • Embryo, Mammalian
  • Female
  • Fibroblasts / metabolism
  • Humans
  • Kinetics
  • Pregnancy
  • Simplexvirus / drug effects
  • Simplexvirus / enzymology
  • Simplexvirus / radiation effects*
  • Ultraviolet Rays*
  • Viral Plaque Assay


  • Diterpenes
  • Aphidicolin
  • DNA Polymerase II