Cloning and sequencing of the xylose isomerase and xylulose kinase genes of Escherichia coli

Appl Environ Microbiol. 1984 Jan;47(1):15-21. doi: 10.1128/aem.47.1.15-21.1984.

Abstract

A 4.2-kilobase-pair fragment of the Escherichia coli chromosome which contains the genes for xylose isomerase and xylulose kinase was cloned into plasmid pBR322. The hybrid plasmid (designated pECX14) complements strains deficient in either or both of the two enzymes. Deletion derivatives of pECX14 were used to localize the two genes on the cloned fragment. The entire nucleotide sequence of the cloned fragment was determined. Open reading frames which, if translated, would encode proteins of molecular weights 54,000 and 52,000 were found. These were identified as the isomerase and kinase structural genes, respectively.

MeSH terms

  • Aldose-Ketose Isomerases*
  • Base Sequence
  • Carbohydrate Epimerases / genetics*
  • Cloning, Molecular*
  • Escherichia coli / enzymology*
  • Escherichia coli / genetics
  • Mutation
  • Phosphotransferases (Alcohol Group Acceptor)*
  • Phosphotransferases / genetics*
  • Plasmids
  • Protein Biosynthesis
  • Transcription, Genetic

Substances

  • Phosphotransferases
  • Phosphotransferases (Alcohol Group Acceptor)
  • xylulokinase
  • Carbohydrate Epimerases
  • Aldose-Ketose Isomerases
  • xylose isomerase

Associated data

  • GENBANK/K01996