Characterization of the replication and stability regions of Agrobacterium tumefaciens plasmid pTAR

J Bacteriol. 1984 Mar;157(3):739-45. doi: 10.1128/jb.157.3.739-745.1984.

Abstract

A 5.4-kilobase region containing the origin of replication and stability maintenance of the 44-kilobase Agrobacterium tumefaciens plasmid pTAR has been mapped and characterized. Within this region is a 1.3-kilobase segment that is capable of directing autonomous replication. The remaining segment contains the stability locus for maintenance of pTAR during nonselective growth. Approximately 35% of pTAR shares sequence homology with pAg119, a 44-kilobase cryptic plasmid in grapevine strain 1D1119. However, no homology was detected between pTAR DNA and several Ti plasmids or several other small cryptic plasmids in many A. tumefaciens strains. A recombinant plasmid containing the origin of replication and stability maintenance region of pTAR was compatible with pTiC58, pTi15955, and pTi119 and incompatible with pAg119. A new compatibility group, Inc Ag-1, is discussed.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Base Sequence
  • DNA Replication*
  • DNA Restriction Enzymes
  • DNA, Bacterial
  • Nucleic Acid Hybridization
  • Plasmids*
  • Rhizobium / genetics*

Substances

  • DNA, Bacterial
  • DNA Restriction Enzymes