Establishment of a Ph1 chromosome-positive cell line from chronic myelogenous leukemia in blast crisis

Int J Cell Cloning. 1983 Jun;1(2):105-17. doi: 10.1002/stem.5530010205.


A new hematopoietic cell line, designated KCL-22, was established in vitro by cultivation of pleural effusion cells obtained from a woman with chronic myelogenous leukemia in blast crisis. KCL-22 grew in suspension culture with a doubling time of 24 h and consisted of immature undifferentiated cells which were positive for periodic acid-Schiff and acid phosphatase staining. Chromosome analysis of the KCL-22 line showed a female karyotype with double Ph1 chromosomes and additional chromosome abnormalities. Its representative karyotype was 52,XX, + 1p-,+6,+8,+8,+8, t(9q+;22q-), +22q-. This cell line possessed receptors for the Fc portion of IgG, but lacked lymphoid cell characteristics and the Epstein-Barr virus-associated nuclear antigen. These results indicate that the KCL-22 cells were derived from chronic myelogenous leukemia cells. This cell line should prove useful for research involving various aspects of chronic myelogenous leukemia.

Publication types

  • Case Reports

MeSH terms

  • Adult
  • Antigens, Viral / analysis
  • Cell Line*
  • Cell Nucleus / ultrastructure
  • Chromosomes, Human, 21-22 and Y*
  • Cytoplasm / ultrastructure
  • DNA Nucleotidylexotransferase / analysis
  • Epstein-Barr Virus Nuclear Antigens
  • Factor VIII / analysis
  • Female
  • Fibrinogen / analysis
  • Herpesvirus 4, Human / immunology
  • Humans
  • Karyotyping
  • Leukemia, Myeloid / analysis
  • Leukemia, Myeloid / genetics
  • Leukemia, Myeloid / pathology*
  • Phagocytosis
  • Pleural Effusion
  • Receptors, Immunologic


  • Antigens, Viral
  • Epstein-Barr Virus Nuclear Antigens
  • Receptors, Immunologic
  • Factor VIII
  • Fibrinogen
  • DNA Nucleotidylexotransferase