Cryo-electron microscopy of viruses

Nature. 1984 Mar 1-7;308(5954):32-6. doi: 10.1038/308032a0.


Thin vitrified layers of unfixed, unstained and unsupported virus suspensions can be prepared for observation by cryo-electron microscopy in easily controlled conditions. The viral particles appear free from the kind of damage caused by dehydration, freezing or adsorption to a support that is encountered in preparing biological samples for conventional electron microscopy. Cryo-electron microscopy of vitrified specimens offers possibilities for high resolution observations that compare favourably with any other electron microscopical method.

MeSH terms

  • Adenoviruses, Human / ultrastructure
  • Bacteriophage lambda / ultrastructure
  • Escherichia coli / ultrastructure
  • Freezing
  • Microscopy, Electron / methods
  • Semliki forest virus / ultrastructure
  • T-Phages / ultrastructure
  • Vesicular stomatitis Indiana virus / ultrastructure
  • Viruses / ultrastructure*