alpha-Noradrenergic potentiation of neurotransmitter-stimulated cAMP production in rat striatal slices

Brain Res. 1984 Feb 13;293(1):57-65. doi: 10.1016/0006-8993(84)91452-5.


The present study examines the possible involvement of alpha-adrenergic receptors in catecholamine-stimulated cAMP production in intact slices of rat striatum. Norepinephrine (NE) produces a greater stimulation of cAMP levels than does the beta-adrenergic agonist, isoproterenol (ISO), and the NE response is inhibited by both the beta-adrenergic antagonist, propranolol, and the alpha-adrenergic antagonist, phentolamine. The alpha-adrenergic agonist, 6-fluoronorepinephrine (6-FNE), has little or no effect on basal cAMP levels; however, 6-FNE causes a marked potentiation of the cAMP response to ISO. Hence, NE stimulation of cAMP levels in striatal slices appears to involve a synergistic interaction between alpha- and beta-adrenergic receptors. alpha-Receptors also potentiate adenosine stimulation of cAMP levels in striatal slices. However, in contrast to results previously reported in cerebral cortical slices, the alpha-adrenergic component of the NE response in striatal slices is not dependent on endogenous adenosine. Finally, 6-FNE interactions with adenylate cyclase in striatal homogenates differ from those observed in the slice preparation. In homogenates, 6-FNE appears to directly stimulate adenylate cyclase through a D-1 receptor. D-1 receptor involvement in catecholamine responses in the striatal slice preparation, on the other hand, appears to be minimal.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Catecholamines / physiology*
  • Corpus Striatum / physiology*
  • Cyclic AMP / biosynthesis*
  • Dose-Response Relationship, Drug
  • In Vitro Techniques
  • Isoproterenol / pharmacology
  • Male
  • Norepinephrine / pharmacology
  • Rats
  • Rats, Inbred Strains
  • Receptors, Adrenergic, alpha / physiology*
  • Receptors, Adrenergic, beta / physiology
  • Receptors, Cell Surface / physiology
  • Receptors, Purinergic


  • Catecholamines
  • Receptors, Adrenergic, alpha
  • Receptors, Adrenergic, beta
  • Receptors, Cell Surface
  • Receptors, Purinergic
  • Cyclic AMP
  • Isoproterenol
  • Norepinephrine