Isolation and characterization of a pleiotropic glucose repression resistant mutant of Saccharomyces cerevisiae

Mol Gen Genet. 1984;193(3):507-12. doi: 10.1007/BF00382091.


A new mutation has been described which confers resistance to catabolite repression in Saccharomyces cerevisiae. The mutant allele, termed grr-1 for glucose repression-resistant, is characterized by insensitivity to glucose repression for the cytoplasmic enzymes invertase, maltase, and galactokinase, as well as the mitochondrial enzyme cytochrome c oxidase. Hexokinase levels in grr-1 mutants are approximately 3-fold higher than the corresponding activity of the parental strain. Although the grr-1 allele is expressed phenotypically similarly to the hex-1 (hxk-2) and hex-2 mutations described by Entian et al. (1977) and Zimmermann and Scheel (1977) respectively, we have shown genetically and physiologically that grr-1 represents a new class of mutation.

MeSH terms

  • Alleles
  • Crosses, Genetic
  • Deoxyglucose / toxicity
  • Electron Transport Complex IV / metabolism
  • Enzyme Repression
  • Galactose / metabolism
  • Genes, Dominant
  • Genes, Fungal
  • Genes, Recessive
  • Genotype
  • Glucose / metabolism*
  • Hexokinase / genetics
  • Mutation*
  • Saccharomyces cerevisiae / drug effects
  • Saccharomyces cerevisiae / genetics*
  • Species Specificity


  • Deoxyglucose
  • Electron Transport Complex IV
  • Hexokinase
  • Glucose
  • Galactose