Identification of a Ty insertion within the coding sequence of the S. cerevisiae URA3 gene

Mol Gen Genet. 1984;193(3):557-60. doi: 10.1007/BF00382100.

Abstract

Insertion mutations in yeast caused by the transposable element Ty have been identified at several genetic loci. In all cases so far, the site of Ty insertion has been in the 5' non-coding region of the affected gene. Experiments presented here demonstrate that the ura3-52 mutation, a non-reverting ura3 mutation, is caused by a Ty insertion mutation within the coding region of the URA3 gene. This Ty insertion within a gene has a similar structure to those in non-coding regions.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Base Sequence
  • DNA Restriction Enzymes
  • DNA Transposable Elements*
  • Genes, Fungal*
  • Mutation*
  • Nucleic Acid Hybridization
  • Saccharomyces cerevisiae / genetics*
  • Uracil / metabolism

Substances

  • DNA Transposable Elements
  • Uracil
  • DNA Restriction Enzymes