The locus of sequence-directed and protein-induced DNA bending

Nature. 1984 Apr 5-11;308(5959):509-13. doi: 10.1038/308509a0.


The bending locus of trypanosome kinetoplast DNA, identified by gel electrophoresis, has tracts of a simple repeat sequence (CA5-6 T) symmetrically distributed about it, with a repeat interval of 10 base pairs. The analogous bending induced when catabolite gene activating protein binds to its recognition sequence near the promoter of the Escherichia coli lac operon is centred on a site about 5-7 base pairs away from the centre of the protein binding site.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Base Composition
  • Base Sequence*
  • Cloning, Molecular
  • DNA Restriction Enzymes
  • DNA, Circular*
  • DNA, Kinetoplast
  • Distamycins
  • Escherichia coli / genetics
  • Lac Operon*
  • Nucleic Acid Conformation*
  • Plasmids
  • Receptors, Cyclic AMP / metabolism*
  • Trypanosoma


  • DNA, Circular
  • DNA, Kinetoplast
  • Distamycins
  • Receptors, Cyclic AMP
  • DNA Restriction Enzymes