Characterization of DNA sequences through which cadmium and glucocorticoid hormones induce human metallothionein-IIA gene

Nature. 1984 Apr 5-11;308(5959):513-9. doi: 10.1038/308513a0.

Abstract

Deletion experiments have defined two stretches of DNA (genetic elements), lying close to the promoter for a human gene for metallothionein, that separately mediate the induction of the gene by heavy metal ions, particularly cadmium, and by glucocorticoid hormones. The element responsible for induction by cadmium is duplicated, yet a single copy is fully functional; the element responsible for induction by glucocorticoid hormones is coincident with the DNA-binding site for the glucocorticoid hormone receptor.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Base Sequence
  • Cadmium / pharmacology*
  • Chromosome Deletion
  • Cloning, Molecular
  • DNA Restriction Enzymes
  • Dexamethasone / pharmacology
  • Genes / drug effects*
  • Glucocorticoids / pharmacology*
  • Humans
  • Metallothionein / genetics*
  • Mutation
  • Operon
  • Receptors, Glucocorticoid / metabolism

Substances

  • Glucocorticoids
  • Receptors, Glucocorticoid
  • Cadmium
  • Dexamethasone
  • Metallothionein
  • DNA Restriction Enzymes

Associated data

  • GENBANK/X00504