DNA polymerase and simian virus 40 infection of resting monkey cells: induction of aphidicolin resistant alpha-polymerase

Nucleic Acids Res. 1983 Dec 10;11(23):8253-68. doi: 10.1093/nar/11.23.8253.


Tightly confluent monkey cell lines BSC-1 and CV-1 held in stale medium for several days exhibited an extremely low level of thymidine incorporation into cellular DNA. Yet, these cells contained a level of alpha-polymerase equal to about 15% of the level in rapidly dividing cells, and they still were capable of supporting replication of SV40 DNA. SV40 infection and culture in stale medium resulted in a four-fold induction of alpha-polymerase in CV-1 cells, whereas no change in alpha-polymerase level was observed in BSC-1 cells. Characterization of alpha-polymerase partially purified from infected CV-1 cells revealed that 80-90% of the enzyme activity was aphidicolin resistant. SV40 DNA replication in resting CV-1 cells, however, was aphidicolin sensitive. SV40 infection of resting CV-1 cells may induce an aphidicolin-resistant enzyme or lead to a modified alpha-polymerase species.

MeSH terms

  • Animals
  • Aphidicolin
  • Cell Line
  • Cell Transformation, Neoplastic*
  • Chlorocebus aethiops
  • DNA Polymerase II / antagonists & inhibitors
  • DNA Polymerase II / genetics*
  • DNA Polymerase II / isolation & purification
  • DNA Replication
  • DNA-Directed DNA Polymerase / isolation & purification
  • Diterpenes / toxicity*
  • Kidney
  • Kinetics
  • Simian virus 40 / enzymology
  • Simian virus 40 / genetics*


  • Diterpenes
  • Aphidicolin
  • DNA Polymerase II
  • DNA-Directed DNA Polymerase