Physical mapping of the ribosomal RNA genes of Mycoplasma capricolum

Nucleic Acids Res. 1984 Mar 12;12(5):2421-6. doi: 10.1093/nar/12.5.2421.


Physical mapping of the rRNA genes of Mycoplasma capricolum was done by digestion of the mycoplasmal DNA with EcoRI, PstI and BglII and hybridization with nick-translated probes consisting of defined portions of the rrnB ribosomal RNA operon of Escherichia coli. The results indicate that the rRNA genes in the chromosome of M. capricolum are arranged in two clusters, each organized in the order 5'-16S-23S-5S-3', resembling the order of the genes in the rrnB operon, with no large spacer regions separating the genes in each cluster.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Base Sequence
  • DNA / genetics*
  • DNA Restriction Enzymes
  • DNA, Ribosomal
  • Escherichia coli / genetics
  • Genes*
  • Genes, Bacterial*
  • Mycoplasma / genetics*
  • Nucleic Acid Hybridization
  • Operon
  • Plasmids
  • Protein Biosynthesis
  • RNA, Ribosomal / genetics*


  • DNA, Ribosomal
  • RNA, Ribosomal
  • DNA
  • DNA Restriction Enzymes