Effect of the serine-borate complex on the relative ability of leukotriene C4, D4 and E4 to inhibit lung and brain [3H] leukotriene D4 and [3H] leukotriene C4 binding: demonstration of the agonists' potency order for leukotriene D4 and leukotriene C4 receptors

Biochem Biophys Res Commun. 1984 Mar 15;119(2):612-7. doi: 10.1016/s0006-291x(84)80293-4.

Abstract

To define the potency order of the leukotrienes for inhibition of [3H] leukotriene D4 and [3H] leukotriene C4 binding, we investigated leukotriene C4, D4 and E4 competition with and without the serine-borate complex in guinea pig lung and brain homogenates. Without it, the rank order of their potency for inhibition of lung [3H] leukotriene D4 or [3H] leukotriene C4 binding was leukotriene C4 = leukotriene D4 greater than leukotriene E4. Presence of the complex altered the potency order for both competition studies: for the [3H] leukotriene D4 competition it was leukotriene D4 greater than leukotriene E4 = leukotriene C4 and for the [3H] leukotriene C4 competition it was leukotriene C4 much greater than leukotriene D4 greater than or equal to leukotriene E4.

MeSH terms

  • Animals
  • Binding, Competitive
  • Borates / pharmacology*
  • Brain / metabolism*
  • Female
  • Guinea Pigs
  • Kinetics
  • Leukotriene E4
  • Lung / metabolism*
  • Receptors, Cell Surface / metabolism*
  • Receptors, Leukotriene
  • SRS-A / analogs & derivatives*
  • SRS-A / metabolism*
  • SRS-A / pharmacology
  • Serine / pharmacology*
  • Tritium

Substances

  • Borates
  • Receptors, Cell Surface
  • Receptors, Leukotriene
  • SRS-A
  • serine-borate complex
  • Tritium
  • Serine
  • Leukotriene E4