Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
, 12 (7), 3219-34

Two Improved Promoter Sequences for the Beta-Lactamase Expression Arising From a Single Base-Pair Substitution

Two Improved Promoter Sequences for the Beta-Lactamase Expression Arising From a Single Base-Pair Substitution

S T Chen et al. Nucleic Acids Res.

Abstract

A mutation in the transposon Tn2660 derived from the plasmid R6K, and resulting in an approximate tenfold increase in penicillin resistance is shown to be a single GC to AT substitution 177 base pairs 'upstream' of the initiation codon of the structural beta-lactamase (bla) gene. This substitution leads to the transcription of two new mRNAs which can be ascribed to the creation of two new overlapping promoter sequences. All the sequences (450bp) examined in the wild-type Tn2660 are identical to those reported in Tn3.

Similar articles

See all similar articles

Cited by 18 articles

See all "Cited by" articles

References

    1. Nature. 1965 Oct 16;208(5007):239-41 - PubMed
    1. Proc Natl Acad Sci U S A. 1983 Jun;80(11):3232-6 - PubMed
    1. J Bacteriol. 1973 Jun;114(3):1116-24 - PubMed
    1. Proc Natl Acad Sci U S A. 1974 Sep;71(9):3455-9 - PubMed
    1. J Bacteriol. 1975 Apr;122(1):250-6 - PubMed

Publication types

Feedback