Two improved promoter sequences for the beta-lactamase expression arising from a single base-pair substitution

Nucleic Acids Res. 1984 Apr 11;12(7):3219-34. doi: 10.1093/nar/12.7.3219.

Abstract

A mutation in the transposon Tn2660 derived from the plasmid R6K, and resulting in an approximate tenfold increase in penicillin resistance is shown to be a single GC to AT substitution 177 base pairs 'upstream' of the initiation codon of the structural beta-lactamase (bla) gene. This substitution leads to the transcription of two new mRNAs which can be ascribed to the creation of two new overlapping promoter sequences. All the sequences (450bp) examined in the wild-type Tn2660 are identical to those reported in Tn3.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Base Composition
  • Base Sequence
  • DNA Restriction Enzymes
  • DNA Transposable Elements
  • DNA-Directed RNA Polymerases / metabolism
  • Escherichia coli / enzymology*
  • Escherichia coli / genetics
  • Operon*
  • Penicillin Resistance
  • Penicillins / toxicity
  • RNA, Messenger / genetics
  • beta-Lactamases / genetics*

Substances

  • DNA Transposable Elements
  • Penicillins
  • RNA, Messenger
  • DNA-Directed RNA Polymerases
  • DNA Restriction Enzymes
  • beta-Lactamases