Recombinant retroviral DNA yielding high expression of hepatitis B surface antigen

EMBO J. 1982;1(12):1573-8. doi: 10.1002/j.1460-2075.1982.tb01357.x.

Abstract

A genomic fragment of hepatitis B virus encoding the surface antigen (HBsAg) was inserted into the proviral genome of Moloney mouse sarcoma virus (MSV), obtained from the mouse cell line G8 -124. The recombinant DNA was introduced into NIH 3T3 mouse fibroblasts. Cells, morphologically transformed by the oncogene of MSV (v-mosM) were selected, established as cell lines and tested for expression of HBsAg. An expression level of up to 4.5 micrograms/10(7) cells/day was detected.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Line
  • Cells, Cultured
  • Cloning, Molecular*
  • DNA Restriction Enzymes
  • DNA, Recombinant / metabolism*
  • DNA, Viral / genetics*
  • Genes*
  • Genes, Viral*
  • Hepatitis B Surface Antigens / genetics*
  • Hepatitis B virus / genetics
  • Hepatitis B virus / immunology*
  • Mice
  • Mice, Inbred Strains
  • Molecular Weight
  • Moloney murine leukemia virus / genetics*
  • Nucleic Acid Hybridization
  • Transfection

Substances

  • DNA, Recombinant
  • DNA, Viral
  • Hepatitis B Surface Antigens
  • DNA Restriction Enzymes