Abstract
A genomic fragment of hepatitis B virus encoding the surface antigen (HBsAg) was inserted into the proviral genome of Moloney mouse sarcoma virus (MSV), obtained from the mouse cell line G8 -124. The recombinant DNA was introduced into NIH 3T3 mouse fibroblasts. Cells, morphologically transformed by the oncogene of MSV (v-mosM) were selected, established as cell lines and tested for expression of HBsAg. An expression level of up to 4.5 micrograms/10(7) cells/day was detected.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Cell Line
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Cells, Cultured
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Cloning, Molecular*
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DNA Restriction Enzymes
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DNA, Recombinant / metabolism*
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DNA, Viral / genetics*
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Genes*
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Genes, Viral*
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Hepatitis B Surface Antigens / genetics*
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Hepatitis B virus / genetics
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Hepatitis B virus / immunology*
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Mice
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Mice, Inbred Strains
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Molecular Weight
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Moloney murine leukemia virus / genetics*
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Nucleic Acid Hybridization
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Transfection
Substances
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DNA, Recombinant
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DNA, Viral
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Hepatitis B Surface Antigens
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DNA Restriction Enzymes