Human Epidermal Growth Factor Receptor cDNA Sequence and Aberrant Expression of the Amplified Gene in A431 Epidermoid Carcinoma Cells

Nature. 1984 May 31-Jun 6;309(5967):418-25. doi: 10.1038/309418a0.

Abstract

The complete 1,210-amino acid sequence of the human epidermal growth factor (EGF) receptor precursor, deduced from cDNA clones derived from placental and A431 carcinoma cells, reveals close similarity between the entire predicted v-erb-B mRNA oncogene product and the receptor transmembrane and cytoplasmic domains. A single transmembrane region of 23 amino acids separates the extracellular EGF binding and cytoplasmic domains. The receptor gene is amplified and apparently rearranged in A431 cells, generating a truncated 2.8-kilobase mRNA which encodes only the extracellular EGF binding domain.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Carcinoma, Squamous Cell
  • Cell Line
  • DNA / metabolism*
  • Epidermal Growth Factor / metabolism
  • ErbB Receptors
  • Gene Amplification*
  • Genes*
  • Humans
  • Nucleic Acid Hybridization
  • Peptide Fragments / analysis
  • RNA, Messenger / genetics
  • Receptors, Cell Surface / genetics*
  • Receptors, Cell Surface / metabolism

Substances

  • Peptide Fragments
  • RNA, Messenger
  • Receptors, Cell Surface
  • Epidermal Growth Factor
  • DNA
  • ErbB Receptors

Associated data

  • GENBANK/K01885
  • GENBANK/K02047
  • GENBANK/X00588
  • GENBANK/X00663