Genetics of herpes simplex virus

J Invest Dermatol. 1984 Jul;83(1 Suppl):42s-47s. doi: 10.1111/1523-1747.ep12281154.


The most direct approach to elucidating the roles of herpes simplex virus (HSV) proteins in the viral replicative cycle has been to isolate temperature-sensitive, cytolysis-resistant, and drug-resistant mutants that exhibit alterations in the synthesis or activity of these proteins. The development of procedures for the introduction of temperature-sensitive mutations into physically defined regions of the viral genome and for fine mapping of these mutations has proven especially valuable. Thus, (1) hydroxylamine mutagenesis of the HSV-1 BglII I fragment (coordinates 0.312-0.415) has facilitated the genetic and functional characterization of the gene for the major viral DNA-binding protein of 130 K molecular weight; (2) the selection of a mutant conditionally able to render infected cells resistant to immune cytolysis has led to identification of an HSV gene involved in the processing of viral glycoproteins; and (3) the combined use of temperature-sensitive and drug-resistant mutants has led to a better definition of the physical limits and functional domains of the gene for HSV DNA polymerase.

Publication types

  • Review

MeSH terms

  • Chromosome Mapping
  • Cloning, Molecular
  • DNA, Viral / genetics
  • DNA-Binding Proteins / genetics
  • DNA-Directed DNA Polymerase / genetics
  • Drug Resistance, Microbial
  • Genes, Viral
  • Genetic Complementation Test
  • Glycoproteins / genetics
  • Mutation
  • Simplexvirus / drug effects
  • Simplexvirus / genetics*
  • Simplexvirus / isolation & purification
  • Temperature
  • Viral Proteins / genetics


  • DNA, Viral
  • DNA-Binding Proteins
  • Glycoproteins
  • Viral Proteins
  • DNA-Directed DNA Polymerase