Light-inducible and chloroplast-associated expression of a chimaeric gene introduced into Nicotiana tabacum using a Ti plasmid vector

Nature. 1984 Jul;310(5973):115-20. doi: 10.1038/310115a0.

Abstract

A chimaeric gene, consisting of the 5'-flanking region of a member of the Pisum sativum gene family encoding ribulose 1,5-bisphosphate carboxylase linked to the coding region of a bacterial chloramphenicol acetyltransferase gene, has been introduced into the genome of the plant Nicotiana tabacum using a Ti plasmid of Agrobacterium tumefaciens. The expression of the chimaeric gene is light-inducible in chloroplast-containing transformed tissue.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Acetyltransferases / genetics*
  • Base Sequence
  • Chimera*
  • Chloramphenicol O-Acetyltransferase
  • Chloroplasts / enzymology*
  • Cloning, Molecular
  • DNA Restriction Enzymes
  • Genes* / radiation effects
  • Genetic Vectors
  • Light
  • Nicotiana / genetics
  • Plants / genetics*
  • Plants, Toxic
  • Plasmids*
  • Rhizobium / genetics*
  • Ribulose-Bisphosphate Carboxylase / genetics*

Substances

  • Acetyltransferases
  • Chloramphenicol O-Acetyltransferase
  • DNA Restriction Enzymes
  • Ribulose-Bisphosphate Carboxylase