Abstract
A chimaeric gene, consisting of the 5'-flanking region of a member of the Pisum sativum gene family encoding ribulose 1,5-bisphosphate carboxylase linked to the coding region of a bacterial chloramphenicol acetyltransferase gene, has been introduced into the genome of the plant Nicotiana tabacum using a Ti plasmid of Agrobacterium tumefaciens. The expression of the chimaeric gene is light-inducible in chloroplast-containing transformed tissue.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Acetyltransferases / genetics*
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Base Sequence
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Chimera*
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Chloramphenicol O-Acetyltransferase
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Chloroplasts / enzymology*
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Cloning, Molecular
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DNA Restriction Enzymes
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Genes* / radiation effects
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Genetic Vectors
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Light
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Nicotiana / genetics
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Plants / genetics*
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Plants, Toxic
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Plasmids*
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Rhizobium / genetics*
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Ribulose-Bisphosphate Carboxylase / genetics*
Substances
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Acetyltransferases
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Chloramphenicol O-Acetyltransferase
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DNA Restriction Enzymes
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Ribulose-Bisphosphate Carboxylase