Mast cell products stimulate collagenase and prostaglandin E production by cultures of adherent rheumatoid synovial cells

Biochem Biophys Res Commun. 1984 Jul 18;122(1):270-6. doi: 10.1016/0006-291x(84)90470-4.


Mast cells were purified from histologically-confirmed dog mastocytomas and extracted for whole mast cell products (MCP). When added to cultures of human adherent rheumatoid synovial cells MCP induced a 50-400 fold increase in prostaglandin E synthesis and a 10-50 fold stimulation of collagenase production. The mast cell stimulatory factor has not been identified and was not due to histamine, heparin or prostaglandin E. These results indicate a novel way in which mast cells might interact with synovial cells to promote the production of inflammatory mediators and proteolytic enzymes which might contribute to connective tissue degradation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Adhesion
  • Cell Extracts / pharmacology*
  • Cells, Cultured
  • Dogs
  • Humans
  • Mast Cells / metabolism*
  • Microbial Collagenase / biosynthesis*
  • Prostaglandins E / biosynthesis*
  • Rheumatic Diseases / metabolism*
  • Synovial Membrane / metabolism*
  • Tissue Extracts / pharmacology*


  • Cell Extracts
  • Prostaglandins E
  • Tissue Extracts
  • Microbial Collagenase