Inactivation of human alpha-1-proteinase inhibitor by gas-phase cigarette smoke

Biochem Biophys Res Commun. 1984 Jul 31;122(2):676-81. doi: 10.1016/s0006-291x(84)80086-8.


Direct exposure of human alpha-1-proteinase inhibitor to the gas-phase smoke from one cigarette results in an initial rapid loss of elastase inhibitory capacity, the amount of which is dependent upon the age of the smoke. This short-term inactivation is not seen when the protein is exposed to aqueous extracts of cigarette smoke (as had been done in the past). Both exposure regimens result in a slow inactivation occurring over several days. We suggest that the short-term inactivation may be due to a peroxynitrate (or a similar reactive species) that is formed from radicals in the gas phase but is unstable in aqueous solution.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Humans
  • Kinetics
  • Pancreatic Elastase / antagonists & inhibitors
  • Peroxides
  • Smoke
  • Smoking*
  • Time Factors
  • alpha 1-Antitrypsin / metabolism*


  • Peroxides
  • Smoke
  • alpha 1-Antitrypsin
  • tert-butyl peroxynitrate
  • Pancreatic Elastase