Production of colony-stimulating factor(s) for granulocyte-macrophage and multipotential (granulocyte/erythroid/megakaryocyte/macrophage) hematopoietic progenitor cells (CFU-GEMM) by clonal lines of human IL-2-dependent T-lymphocytes

Exp Hematol. 1984 Oct;12(9):720-7.

Abstract

Human T-lymphocyte lines that were selected for recognition of HLA-DR6 antigen and were dependent for growth in vitro on an added source of interleukin-2 (IL-2) were derived from the peripheral blood of normal individuals. Each was tested for production of a lymphokine(s) with properties of granulocyte-macrophage colony-stimulating factor (GM-CSF) using as target cells nonadherent cells from human long-term bone marrow cultures (LTBMC) or fresh marrow. Each of eight T-lymphocyte lines that were OKT3, OKT4, and HLA-DR positive produced GM-CSF that stimulated colony formation by both LTBMC cells and fresh marrow. Individually examined single-cell-derived bone marrow colonies growing in T-cell GM-CSF contained peroxidase-positive neutrophils, and macrophage-monocytes (GM-CFUc). Supernatant from a single-cell-derived T-cell clonal line designated F1 stimulated formation of granulocyte-macrophage colonies, megakaryocyte colonies, macroscopic erythroid bursts, and multipotential colonies containing erythroid cells, megakaryocytes, neutrophilic and eosinophilic granulocytes, and monocyte-macrophages (CFU-GEMM) in the presence of added erythropoietin. These data indicate that human IL-2-responsive T-lymphocytes produce lymphokine(s) that stimulate proliferation of primitive as well as committed hematopoietic stem cells, and implicate human T-lymphocytes in regulation of human multipotential hematopoietic stem cells in vivo.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Antigens, Surface / analysis
  • Bone Marrow Cells*
  • Cell Division
  • Cell Line
  • Cells, Cultured
  • Clone Cells
  • Colony-Forming Units Assay
  • Colony-Stimulating Factors / biosynthesis*
  • Colony-Stimulating Factors / physiology
  • Erythrocytes / cytology*
  • Granulocytes / cytology*
  • Hematopoietic Stem Cells / cytology*
  • Humans
  • Interleukin-2 / physiology*
  • Macrophages / cytology*
  • Megakaryocytes / cytology*
  • T-Lymphocytes / immunology
  • T-Lymphocytes / physiology*

Substances

  • Antigens, Surface
  • Colony-Stimulating Factors
  • Interleukin-2