Cloning and expression of cDNA for human lymphotoxin, a lymphokine with tumour necrosis activity

Nature. 1984 Dec 20-1985 Jan 2;312(5996):721-4. doi: 10.1038/312721a0.


A chemically-synthesized gene and natural complementary DNA coding for human lymphotoxin were isolated and engineered for expression in Escherichia coli. Purified recombinant lymphotoxin shows cytotoxic activity on murine and human tumour cell lines in vitro and causes necrosis of certain murine sarcomas in vivo.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cells, Cultured
  • Cloning, Molecular
  • DNA / genetics
  • Gene Expression Regulation
  • Humans
  • Lymphokines / genetics
  • Lymphotoxin-alpha / genetics*
  • Lymphotoxin-alpha / therapeutic use
  • Mice
  • Sarcoma, Experimental / therapy


  • Lymphokines
  • Lymphotoxin-alpha
  • DNA

Associated data

  • GENBANK/X01393