Abstract
Escherichia coli mutants lacking exonuclease III (xthA) are exceptionally sensitive to hydrogen peroxide. They are killed by H2O2 at 20 times the rate of wild-type bacteria and at 3 to 4 times the rate of recA cells. This is the first clear phenotypic sensitivity reported for xth- E. coli and should aid in clarifying peroxide-induced lethality and the in vivo role of exonuclease III.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Catalase / metabolism
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Escherichia coli / drug effects*
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Escherichia coli / enzymology
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Escherichia coli / genetics
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Exodeoxyribonucleases / physiology*
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Hydrogen Peroxide / pharmacology*
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Mutation
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Phenotype
Substances
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Hydrogen Peroxide
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Catalase
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Exodeoxyribonucleases
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exodeoxyribonuclease III