Abstract
Chromosomal DNA from strain UT400, a previously described deletion mutant of Escherichia coli K-12 that lacks outer membrane protein a, failed to hybridize with plasmid DNA (pGGC110) containing the structural gene for protein a. We designate the genetic locus for protein a, located at approximately 12.5 min of the E. coli chromosome, ompT.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Bacterial Outer Membrane Proteins
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Chromosome Mapping
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Chromosomes, Bacterial*
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Escherichia coli / genetics*
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Genes*
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Membrane Proteins / genetics*
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Nucleic Acid Hybridization
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Plasmids
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Temperature
Substances
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Bacterial Outer Membrane Proteins
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Membrane Proteins