Use of Aspergillus fumigatus mycelial antigens in enzyme-linked immunosorbent assay and counter-immunoelectrophoresis

J Med Microbiol. 1983 Feb;16(1):97-105. doi: 10.1099/00222615-16-1-97.

Abstract

Antigens obtained from the ruptured mycelium of Aspergillus fumigatus were separated on the basis of their attachment or non-attachment to Concanavalin A-linked Sepharose gel. They were analysed by double-diffusion and two-dimensional immunoelectrophoresis with rabbit antiserum and sera from patients with aspergillus-related disease. Their sensitivity was assessed in an enzyme-linked immunosorbent assay (ELISA) for immunoglobulin G. Sera which gave very low or high extinction readings on ELISA gave, in general, comparable results on counter-immunoelectrophoresis; but weak electrophoretic reactions were not closely paralleled by ELISA results. The unfractionated extract and the fraction bound by Concanavalin A-Sepharose had similar ability to detect specific IgG in sera from patients with aspergillosis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigens, Fungal / immunology*
  • Aspergillosis / immunology
  • Aspergillus fumigatus / immunology*
  • Counterimmunoelectrophoresis*
  • Enzyme-Linked Immunosorbent Assay*
  • Humans
  • Immune Sera
  • Immunoelectrophoresis*
  • Immunoenzyme Techniques*
  • Immunoglobulin G / immunology

Substances

  • Antigens, Fungal
  • Immune Sera
  • Immunoglobulin G