Rat myoblast fusion requires metalloendoprotease activity

Cell. 1983 Jan;32(1):257-65. doi: 10.1016/0092-8674(83)90516-0.


The calcium-dependent fusion of cultured rat myoblasts to multinucleate myotubes appears to require the activity of a neutral metalloendoprotease at the time of fusion. Metalloendoprotease inhibitors and synthetic dipeptide substrates prevent myoblast fusion when added to fusion-competent myoblasts with the addition of calcium. Metalloendoprotease activity has been identified and partially characterized in myoblast membranes with a fluorogenic protease substrate, and is inhibited by the same compounds that prevent fusion.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Cell Fusion / drug effects*
  • Cells, Cultured
  • Metalloendopeptidases
  • Muscles / cytology*
  • Muscles / enzymology
  • Protease Inhibitors* / pharmacology*
  • Rats


  • Protease Inhibitors
  • Metalloendopeptidases