Monoclonal antibodies to human glomerular cells: a marker for glomerular epithelial cells

Nephron. 1983;33(2):83-90. doi: 10.1159/000182918.


A monoclonal antibody, PHM 5, directed against human glomerular epithelial cells, was prepared after immunisation of a mouse with isolated human glomeruli and fusion of spleen cells with a mouse myeloma. Binding of antibody to isolated glomeruli was detected by radioimmune indirect binding assay, and specificity for glomerular epithelial cells was shown using a four-layer peroxidase-antiperoxidase technique applied to epoxy resin sections of the human kidney cortex. PHM 5 appears to detect a human-specific cell surface carbohydrate antigen not previously described, and can be used to identify epithelial cells in renal biopsy sections and in culture outgrowths of isolated glomeruli.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal / immunology*
  • Antibodies, Monoclonal / isolation & purification
  • Antibody Specificity
  • Antigens, Surface / immunology*
  • Binding Sites, Antibody
  • Epithelium / immunology
  • Humans
  • Hybridomas
  • Immunoenzyme Techniques
  • Kidney Glomerulus / immunology*
  • Mice


  • Antibodies, Monoclonal
  • Antigens, Surface