Baculovirus replication: inhibition of Trichoplusia ni multiple nuclear polyhedrosis virus by [E]-5-(2-bromovinyl)-2'-deoxyuridine

J Gen Virol. 1983 Jun;64 (Pt 6):1221-7. doi: 10.1099/0022-1317-64-6-1221.

Abstract

[E]-5-(2-bromovinyl)-2'-deoxyuridine (BVdU) inhibits the replication of the baculovirus Trichoplusia ni multiple nucleocapsid nuclear polyhedrosis virus in Spodoptera frugiperda cells. Virus-specific DNA synthesis and late protein synthesis are suppressed by the drug. BVdU is phosphorylated by deoxythymidine (deoxycytidine) kinase present in both uninfected and virus-infected cells, and in its 5'-triphosphate form it inhibits DNA polymerase activity in virus-infected cells. The effect of the BVdU is not completely reversible. Phosphonoacetic acid, phosphonoformic acid and Acyclovir have no effect on baculovirus replication. Acyclovir fails to compete with deoxycytidine and thymidine as substrates for pyrimidine deoxynucleoside kinase in virus-infected and uninfected cells.

MeSH terms

  • Animals
  • Antiviral Agents / pharmacology*
  • Bromodeoxyuridine / analogs & derivatives*
  • Bromodeoxyuridine / pharmacology
  • DNA Replication / drug effects*
  • DNA-Directed DNA Polymerase / metabolism
  • Deoxycytidine Kinase / metabolism
  • Insect Viruses / drug effects
  • Insect Viruses / enzymology
  • Insect Viruses / genetics*
  • Lepidoptera
  • Thymidine Kinase / metabolism
  • Virus Replication / drug effects

Substances

  • Antiviral Agents
  • brivudine
  • Thymidine Kinase
  • Deoxycytidine Kinase
  • DNA-Directed DNA Polymerase
  • Bromodeoxyuridine