Compilation and analysis of Escherichia coli promoter DNA sequences

Nucleic Acids Res. 1983 Apr 25;11(8):2237-55. doi: 10.1093/nar/11.8.2237.


The DNA sequence of 168 promoter regions (-50 to +10) for Escherichia coli RNA polymerase were compiled. The complete listing was divided into two groups depending upon whether or not the promoter had been defined by genetic (promoter mutations) or biochemical (5' end determination) criteria. A consensus promoter sequence based on homologies among 112 well-defined promoters was determined that was in substantial agreement with previous compilations. In addition, we have tabulated 98 promoter mutations. Nearly all of the altered base pairs in the mutants conform to the following general rule: down-mutations decrease homology and up-mutations increase homology to the consensus sequence.

Publication types

  • Research Support, U.S. Gov't, P.H.S.
  • Review

MeSH terms

  • Base Sequence*
  • DNA, Bacterial / genetics*
  • DNA-Directed RNA Polymerases / metabolism
  • Escherichia coli / genetics*
  • Mutation
  • Operon*


  • DNA, Bacterial
  • DNA-Directed RNA Polymerases