The Role of Zinc in 5',5'-diadenosine Tetraphosphate Production by Aminoacyl-Transfer RNA Synthetases

Mol Cell Biochem. 1983;52(1):3-11. doi: 10.1007/BF00230583.


Aminoacyl-tRNA synthetases are capable of converting 5'-ATP into 5',5'-diadenosine tetraphosphate. The reaction reflects the reversal of enzyme-bound aminoacyl-adenylate by ATP instead of PPi. In the case of a few prokaryotic as well as eukaryotic aminoacyl-tRNA synthetases, the initial rate of diadenosine tetraphosphate synthesis can be greatly enhanced upon adding small amounts of zinc. This observation enables us to establish a relationship between diadenosine tetraphosphate, a nucleotide possibly involved in controlling cell proliferation, and a metallic cofactor, which is believed to play a role in tumour growth.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenine Nucleotides / metabolism*
  • Amino Acyl-tRNA Synthetases / metabolism*
  • Cadmium / pharmacology
  • Chromatography, High Pressure Liquid
  • Dinucleoside Phosphates*
  • Escherichia coli / enzymology
  • Zinc / pharmacology*


  • Adenine Nucleotides
  • Dinucleoside Phosphates
  • Cadmium
  • diadenosine tetraphosphate
  • Amino Acyl-tRNA Synthetases
  • Zinc