Peptidyltransferase center of ribosomes. On the mechanism of action of alkaloid lycorine

FEBS Lett. 1983 Aug 22;160(1-2):129-33. doi: 10.1016/0014-5793(83)80951-x.

Abstract

The molecular mechanism of action of the alkaloid lycorine has been revised. According to our results, lycorine inhibits the binding of CACCA-Leu comes from Ac to the donor site of the peptidyltransferase center of wheat-germ ribosomes, whereas the transpeptidation reaction in the system with the minimal model donor is not inhibited. The equilibrium constant of CACCA-Leu comes from Ac to the donor site of 80 S ribosomes is measured.

Publication types

  • Comparative Study

MeSH terms

  • Acyltransferases / metabolism*
  • Amaryllidaceae Alkaloids*
  • Escherichia coli / enzymology
  • Peptidyl Transferases / metabolism*
  • Phenanthridines / pharmacology*
  • Plants / enzymology
  • Protein Binding
  • RNA, Transfer, Amino Acyl / metabolism
  • Ribosomes / enzymology*
  • Species Specificity
  • Triticum / enzymology

Substances

  • Amaryllidaceae Alkaloids
  • Phenanthridines
  • RNA, Transfer, Amino Acyl
  • Acyltransferases
  • Peptidyl Transferases
  • lycorine