Construction of artificial chromosomes in yeast

Nature. 1983 Sep 15-21;305(5931):189-93. doi: 10.1038/305189a0.


Fifty-five-kilobase long artificial chromosomes containing cloned genes, replicators, centromeres and telomeres have been constructed in yeast. These molecules have many of the properties of natural yeast chromosomes. Centromere function is impaired on short (less than 20 kilobases) artificial chromosomes.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Centromere / physiology
  • Chromosomes / physiology*
  • Cloning, Molecular
  • DNA Replication
  • Genetic Engineering*
  • Meiosis
  • Mitosis
  • Plasmids
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae / ultrastructure