Differential localization of two myosins within nematode thick filaments

Cell. 1983 Sep;34(2):477-90. doi: 10.1016/0092-8674(83)90381-1.

Abstract

The body wall muscle cells of the nematode, Caenorhabditis elegans, contain two unique types of myosin heavy chain, A and B. We have utilized an immunochemical approach to define the structural location of these two myosins within body wall muscle thick filaments. By immunofluorescence microscopy, myosin B antibodies label the thick filament-containing A-bands of body wall muscle with the exception of a thin gap at the center of each A-band, and myosin A antibodies react to form a medial fluorescent stripe within each A-band. The complexes of these monoclonal antibodies with isolated thick filaments were negatively stained and studied by electron microscopy. The myosin B antibody reacts with the polar regions of all filaments but does not react with a central 0.9 micron zone. The myosin A antibody reacts with a central 1.8 micron zone in all filaments but does not react with the polar regions.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antibodies, Monoclonal
  • Caenorhabditis
  • Fluorescent Antibody Technique
  • Microscopy, Electron
  • Muscles / analysis*
  • Myosins / analysis*
  • Myosins / immunology
  • Tissue Distribution

Substances

  • Antibodies, Monoclonal
  • Myosins