Plasmid-mediated adhesion in enteropathogenic Escherichia coli

J Pediatr Gastroenterol Nutr. 1983;2(3):534-8. doi: 10.1097/00005176-198302030-00023.


A survey of classical serotype enteropathogenic Escherichia coli has been made with respect to their plasmid profile and ability to adhere to HEp-2 cells. Thirty-one of the 32 strains examined contained a 50-70 Md plasmid, and many exhibited HEp-2 adherence. Strain E2348 (0127:H6), which causes diarrhea in volunteers and is HEp-2-adhesive, was chosen for further study. The large 55 Md plasmid in E2348, pMAR2, has been marked with a transposon coding for ampicillin resistance. E2348 that has been cured of pMAR2 loses the ability to adhere to HEp-2 cells, while HB101, a nonadherent E. coli K12, acquires HEp-2 adhesiveness after gaining this plasmid. Plasmid presence was also shown to correlate with in vivo adhesion to intestine, using the colostrum-deprived piglet model.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Animals, Newborn
  • Cells, Cultured
  • Escherichia coli / genetics*
  • Escherichia coli / pathogenicity
  • Genes, Bacterial
  • In Vitro Techniques
  • Intestinal Mucosa / cytology
  • Plasmids*
  • Swine
  • Virulence