Qualitative patterns of protein synthesis in the mouse oocyte

J Exp Zool. 1976 Dec;198(3):337-42. doi: 10.1002/jez.1401980306.

Abstract

Mouse oocytes were found to synthesize proteins actively at the germinal vesicle, metaphase I, metaphase II, and pronuclear (6 hours post-fertilization) stages. The qualitative pattern components being synthesized in vitro, as demonstrated using polyacrylamide gel electrophoresis, changed throughout maturation and fertilization. Oocytes were arrested at metaphase I by greater than 0.1 mug/ml cycloheximide or actinomycin D. The protein pattern in oocytes cultured in the presence of actinomycin D progresses to a metaphase II pattern in spite of the nuclear maturation arrest, indicating a dissociation between meiotic maturation and the changes in the pattern of proteins synthesized at different stages of maturation.

MeSH terms

  • Animals
  • Cycloheximide / pharmacology
  • Dactinomycin / pharmacology
  • Female
  • Fertilization
  • Male
  • Meiosis
  • Mice
  • Oocytes / cytology
  • Oocytes / metabolism*
  • Ovum / metabolism*
  • Protein Biosynthesis*

Substances

  • Dactinomycin
  • Cycloheximide