Lack of endotoxin in Borrelia hispanica and Treponema pallidum

Proc Soc Exp Biol Med. 1983 Oct;174(1):47-52. doi: 10.3181/00379727-174-41702.


Borrelia hispanica from infected guinea pigs and Treponema pallidum from testicular syphilomas of rabbits were assayed for the presence of endotoxin with the Limulus lysate test. A suspension of Borrelia, containing 1.3 X 10(8) spirochetes/ml, was nonreactive both when it was tested as intact organisms, and when tested after disruption of the spirochetes by sonication. Eight different suspensions of treponemes, ranging from 0.6 X 10(9) to 3 X 10(9) treponemes/ml, were negative at a 1:10 dilution and were no more active than control suspensions of normal rabbit testes. Therefore, it was concluded that T. pallidum, as well as the Borrelia, possessed no endotoxin.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Borrelia / analysis*
  • Endotoxins / analysis*
  • Guinea Pigs
  • Limulus Test
  • Male
  • Rabbits
  • Sonication
  • Testis / analysis
  • Treponema pallidum / analysis*


  • Endotoxins