A selective medium for the two major subgroups of the bacterium Streptococcus mutans isolated from human dental plaque and saliva

Arch Oral Biol. 1983;28(7):599-603. doi: 10.1016/0003-9969(83)90007-9.

Abstract

A selective medium was developed on which both major subgroups of Strep. mutans (c/e/f and d/g) can be cultured and recognized on the basis of colonial morphology, Trypticase, yeast, cystine (TYC) agar was modified by adding separately autoclaved sucrose to a final concentration of 20 per cent and 0.1 unit/ml bacitracin (TYCSB). This medium yielded significantly higher counts of Strep. mutans than the widely used mitis-salivarius bacitracin (MSB) medium.

Publication types

  • Comparative Study

MeSH terms

  • Bacitracin / metabolism
  • Bacteriological Techniques*
  • Culture Media
  • Dental Plaque / microbiology*
  • Humans
  • Saliva / microbiology*
  • Streptococcus mutans / isolation & purification*
  • Streptococcus mutans / metabolism
  • Sucrose / metabolism

Substances

  • Culture Media
  • Bacitracin
  • Sucrose