Isolation and purification of morphogenic autoregulatory substance produced by Candida albicans

J Biochem. 1983 Sep;94(3):777-83. doi: 10.1093/oxfordjournals.jbchem.a134419.


The human pathogenic, dimorphic fungus Candida albicans produces in vitro a factor designated morphogenic autoregulatory substance (MARS) which suppresses yeast to mycelial transition. MARS is best produced when a high concentration of yeast cells (2 X 10(8)/ml) in a chemically defined medium is incubated at 37 degrees C for 9-15 h. MARS was isolated to apparent homogeneity by the following successive steps: charcoal absorption; pyridine elution; ether extraction; high pressure liquid chromatography, and Sephadex LH-20 chromatography. A 469-fold increase in purity was obtained by these procedures. The use of purified MARS to study C. albicans yeast to mycelial transition may lead to a better understanding of morphogeneis of this important opportunistic pathogen.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Candida albicans / growth & development*
  • Chromatography, High Pressure Liquid
  • Chromatography, Thin Layer
  • Growth Substances / isolation & purification*
  • Kinetics


  • Growth Substances