The accuracy of poly(U) translation by different eukaryotic tRNAs

FEBS Lett. 1983 Nov 28;164(1):93-6. doi: 10.1016/0014-5793(83)80026-x.

Abstract

In this work we present comparative data on rates of phenylalanine and leucine incorporation into the poly(U) dependent product of cell-free translation by different eukaryotic tRNAs at high Mg2+ concentration. The frequency of translation errors has been found to depend upon the value of the tRNAPhe:tRNALeu ratio and the peculiarities of isoacceptor tRNAsLeu of different origin.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Carbon Radioisotopes
  • Cell Differentiation
  • Female
  • Kinetics
  • Leucine / metabolism
  • Liver / metabolism*
  • Mammary Glands, Animal / metabolism*
  • Phenylalanine / metabolism
  • Plants / metabolism
  • Poly U / genetics*
  • Protein Biosynthesis*
  • RNA, Transfer / genetics*
  • Rabbits
  • Saccharomyces cerevisiae / metabolism*
  • Species Specificity
  • Triticum / metabolism

Substances

  • Carbon Radioisotopes
  • Poly U
  • Phenylalanine
  • RNA, Transfer
  • Leucine