Abstract
We placed the Saccharomyces cerevisiae GAL4 gene under control of the galactose regulatory system by fusing it to the S. cerevisiae GAL1 promoter. After induction with galactose, GAL4 is now transcribed at about 1,000-fold higher levels than in wild-type S. cerevisiae. This regulated high-level expression has enabled us to tentatively identify two GAL4-encoded proteins.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Base Sequence
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Culture Media
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Fungal Proteins / biosynthesis
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Fungal Proteins / genetics*
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Galactose / metabolism
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Genes, Fungal*
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Operon
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Plasmids
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Protein Biosynthesis
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RNA, Fungal / isolation & purification
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Saccharomyces cerevisiae / genetics*
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Saccharomyces cerevisiae / metabolism
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Transcription, Genetic
Substances
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Culture Media
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Fungal Proteins
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RNA, Fungal
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Galactose