Mutagenic specificity of a novel T4 DNA polymerase mutant

Genetics. 1984 Mar;106(3):335-45. doi: 10.1093/genetics/106.3.335.


The in vivo mutational specificity of a novel T4 DNA polymerase mutator mutant, tsM19, was determined. Two genetic tester systems were used to characterize the mutant. Results of our studies indicate that tsM19 promotes transition and transversion mutagenesis and, possibly, frameshift mutagenesis. Central G:C base pairs in runs of three or more consecutive G:C base pairs may be target sites for tsM19-induced transitions.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA-Directed DNA Polymerase / genetics*
  • DNA-Directed DNA Polymerase / metabolism
  • Escherichia coli / enzymology*
  • Escherichia coli / genetics
  • Mutation*
  • T-Phages / enzymology*
  • T-Phages / genetics


  • DNA-Directed DNA Polymerase