Molecular cloning and characterization of the RAD1 gene of Saccharomyces cerevisiae

Gene. 1983 Dec;26(2-3):119-26. doi: 10.1016/0378-1119(83)90181-6.

Abstract

We have cloned the RAD1 gene of Saccharomyces cerevisiae and physically mapped it to a 4.0-kb DNA fragment from chromosome XVI. The RAD1 gene determines a transcript of 3.1 kb, and the direction of transcription was found to be leftwards, from EcoRI towards Bg/II (Fig. 1). Deletions of the RAD1 gene were made and were found to have no effect on viability of vegetative cells or spores, or on sporulation.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Chromosome Mapping
  • Cloning, Molecular
  • DNA Repair / radiation effects
  • DNA, Fungal / genetics
  • Fungal Proteins / genetics
  • Genes, Fungal*
  • Mutation
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae / radiation effects
  • Transcription, Genetic

Substances

  • DNA, Fungal
  • Fungal Proteins