Beta 2 microglobulin-like polypeptide of the goldfish, Carassius auratus

Mol Immunol. 1984 Jan;21(1):105-8. doi: 10.1016/0161-5890(84)90095-6.

Abstract

Antisera to human beta 2 microglobulin (beta 2M) detected a plasma membrane molecule on goldfish (Carassius auratus) cells in immunofluorescence. A goldfish molecule detected by radioimmunoassay (RIA) co-eluted with human beta 2M on gel filtration. By affinity chromatography on immobilized antibody to human beta 2M, a molecule was purified (from extracts of goldfish) that showed, on SDS-polyacrylamide gel electrophoresis, a mobility similar to that of human beta 2M (apparent Mr 12,800 +/- 500).

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Chromatography, Gel
  • Cross Reactions
  • Cyprinidae / metabolism*
  • Electrophoresis, Polyacrylamide Gel
  • Female
  • Goldfish / metabolism*
  • Male
  • Membrane Proteins / analysis
  • Radioimmunoassay
  • beta 2-Microglobulin / immunology
  • beta 2-Microglobulin / isolation & purification*

Substances

  • Membrane Proteins
  • beta 2-Microglobulin