Is intracellular Ca2+ the trigger for oxygen radical production by polymorphonuclear leucocytes?

Cell Calcium. 1984 Feb;5(1):1-19. doi: 10.1016/0143-4160(84)90150-7.


The aim of this paper is critically to evaluate the existing evidence for the role of intracellular Ca2+ in polymorphonuclear leucocyte (PMN) activation and in particular in oxygen radical production. Indirect experiments are based on the manipulation of extracellular Ca2+, measurement of 45Ca fluxes, employing pharmacological agents such as Ca2+-ionophores and intracellular Ca2+ antagonists and monitoring chlortetracycline fluorescence. Experiments of this type do not provide the necessary definitive evidence that an increase in intracellular Ca2+ is the trigger for PMN activation. Recent direct measurements of intracellular free Ca2+ using the Ca2+-activated photoprotein, obelin, and the Ca2+-sensitive fluorescent indicator, quin 2, have provided evidence for the existence of two distinct mechanisms of activation, one triggered by a rise in intracellular Ca2+ and the other independent of a rise in intracellular Ca2+. The source of the Ca2+ for the former mechanism is mainly extracellular but can also come from an intracellular Ca2+ store.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Aminoquinolines
  • Animals
  • Calcium / physiology*
  • Calcium Channel Blockers
  • Calcium Radioisotopes
  • Chlortetracycline / pharmacology
  • Fluorescent Dyes
  • Free Radicals*
  • Histocytochemistry
  • Humans
  • Ionophores
  • Luminescent Proteins / metabolism
  • Neutrophils / metabolism*
  • Oxygen / metabolism*
  • Subcellular Fractions / metabolism


  • Aminoquinolines
  • Calcium Channel Blockers
  • Calcium Radioisotopes
  • Fluorescent Dyes
  • Free Radicals
  • Ionophores
  • Luminescent Proteins
  • obelin
  • Quin2
  • Oxygen
  • Calcium
  • Chlortetracycline