Presence of HLA-D/DR antigens on the membrane of breast tumour cells

Clin Exp Immunol. 1984 Apr;56(1):215-21.


HLA-D/DR (Ia) glycoproteins were identified in human breast carcinoma and normal mammary gland cells by means of an anti-Ia monoclonal antibody. Two techniques were used: (1) immunoperoxidase staining performed on histological sections and (2) Ia glycoproteins were isolated as follows: firstly by radioactive labelling of isolated cells, then by filtration on Sephadex G25, followed by Lens culinaris chromatography, and immune complex formation and then elution on protein A-Sepharose. Lastly, the immune complex was studied by chromatofocusing. Both techniques revealed that Ia expression was found in carcinoma cells, but not in normal cells.

MeSH terms

  • Adenofibroma / immunology
  • Antibodies, Monoclonal / immunology
  • Antigen-Antibody Complex / isolation & purification
  • Breast / immunology
  • Breast Neoplasms / immunology*
  • Female
  • Glycoproteins / isolation & purification
  • HLA-DR Antigens
  • Histocompatibility Antigens Class II / analysis*
  • Histocompatibility Antigens Class II / isolation & purification
  • Humans
  • Immunoenzyme Techniques


  • Antibodies, Monoclonal
  • Antigen-Antibody Complex
  • Glycoproteins
  • HLA-DR Antigens
  • Histocompatibility Antigens Class II