Correlation of DNA adenine methylase activity with spontaneous mutability in Escherichia coli K-12

Gene. 1984 Apr;28(1):123-5. doi: 10.1016/0378-1119(84)90095-7.

Abstract

Using a multicopy plasmid in which the tac promoter has been placed in front of the dam gene of Escherichia coli K-12, we show that levels of DNA adenine methylase activity are correlated with the spontaneous mutation frequency.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Bacterial Proteins / genetics*
  • Bacterial Proteins / physiology
  • DNA Repair
  • DNA, Bacterial / metabolism
  • Escherichia coli / genetics*
  • Methyltransferases / genetics*
  • Methyltransferases / physiology
  • Mutation*
  • Plasmids
  • Site-Specific DNA-Methyltransferase (Adenine-Specific)

Substances

  • Bacterial Proteins
  • DNA, Bacterial
  • Methyltransferases
  • Site-Specific DNA-Methyltransferase (Adenine-Specific)