Isolation of the beta-tubulin gene from yeast and demonstration of its essential function in vivo

Cell. 1983 May;33(1):211-9. doi: 10.1016/0092-8674(83)90350-1.


A DNA fragment from yeast (Saccharomyces cerevisiae) was identified by its homology to a chicken beta-tubulin cDNA and cloned. The fragment was shown to be unique in the yeast genome and to contain the gene for yeast beta-tubulin, since it can complement a benomyl-resistant conditional-lethal mutation. A smaller subfragment, when used to direct integration of a plasmid to the benomyl resistance locus in a diploid cell, disrupted one of the beta-tubulin genes and concomitantly created a recessive lethal mutation, indicating that the single beta-tubulin gene of yeast has an essential function. Determination of the nucleotide sequence reveals extensive amino acid sequence homology (more than 70%) between yeast and chicken brain beta-tubulins.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Chickens / genetics
  • DNA, Fungal / genetics
  • Genes
  • Genes, Lethal
  • Mutation
  • Saccharomyces cerevisiae / genetics
  • Tubulin / genetics*


  • DNA, Fungal
  • Tubulin

Associated data

  • GENBANK/J01384