A novel repair enzyme: UVRABC excision nuclease of Escherichia coli cuts a DNA strand on both sides of the damaged region
- PMID: 6380755
- DOI: 10.1016/0092-8674(83)90354-9
A novel repair enzyme: UVRABC excision nuclease of Escherichia coli cuts a DNA strand on both sides of the damaged region
Abstract
The uvrA, uvrB, and uvrC proteins of Escherichia coli were purified from strains that greatly overproduce these proteins. Using the purified proteins, the UVRABC nuclease was reconstituted in vitro. The reconstituted enzyme acted specifically on DNA damaged with UV, cis-platinum, and psoralen plus near UV. When UV-irradiated DNA was used as substrate, the enzyme made two cuts on the damaged DNA strand, one on each side of the damaged region. The enzyme hydrolyzed the eighth phosphodiester bond on the 5' side of pyrimidine dimers. On the 3' side of pyrimidine dimers, the UVRABC nuclease cut the fourth or the fifth phosphodiester bond 3' to pyrimidine dimers. The oligonucleotide with the damaged bases that is generated by these two cuts was released during treatment with the enzyme. We have also obtained evidence suggesting that the enzyme acts by the same mechanism on PydC photoproducts which are thought to be of primary importance in UV-induced mutagenesis.
Similar articles
-
The repair of psoralen monoadducts by the Escherichia coli UvrABC endonuclease.Nucleic Acids Res. 1987 Jun 25;15(12):4957-71. doi: 10.1093/nar/15.12.4957. Nucleic Acids Res. 1987. PMID: 3299260 Free PMC article.
-
Escherichia coli DNA photolyase stimulates uvrABC excision nuclease in vitro.Proc Natl Acad Sci U S A. 1984 Dec;81(23):7397-401. doi: 10.1073/pnas.81.23.7397. Proc Natl Acad Sci U S A. 1984. PMID: 6390436 Free PMC article.
-
Reactions of the UVRABC excision nuclease with DNA damaged by diamminedichloroplatinum(II).Nucleic Acids Res. 1985 Oct 25;13(20):7395-412. doi: 10.1093/nar/13.20.7395. Nucleic Acids Res. 1985. PMID: 3903663 Free PMC article.
-
Structure and function of the (A)BC excinuclease of Escherichia coli.Mutat Res. 1990 Sep-Nov;236(2-3):203-11. doi: 10.1016/0921-8777(90)90005-p. Mutat Res. 1990. PMID: 2204825 Review.
-
Nucleotide excision repair in Escherichia coli.Microbiol Rev. 1990 Mar;54(1):18-51. doi: 10.1128/mr.54.1.18-51.1990. Microbiol Rev. 1990. PMID: 2181258 Free PMC article. Review.
Cited by
-
Prokaryotic nucleotide excision repair.Cold Spring Harb Perspect Biol. 2013 Mar 1;5(3):a012591. doi: 10.1101/cshperspect.a012591. Cold Spring Harb Perspect Biol. 2013. PMID: 23457260 Free PMC article. Review.
-
ATPase activity of the UvrA and UvrAB protein complexes of the Escherichia coli UvrABC endonuclease.Nucleic Acids Res. 1989 Jun 12;17(11):4145-59. doi: 10.1093/nar/17.11.4145. Nucleic Acids Res. 1989. PMID: 2525700 Free PMC article.
-
Involvement of helicase II (uvrD gene product) and DNA polymerase I in excision mediated by the uvrABC protein complex.Proc Natl Acad Sci U S A. 1985 Aug;82(15):4925-9. doi: 10.1073/pnas.82.15.4925. Proc Natl Acad Sci U S A. 1985. PMID: 3161077 Free PMC article.
-
Escherichia coli Fpg protein and UvrABC endonuclease repair DNA damage induced by methylene blue plus visible light in vivo and in vitro.J Bacteriol. 1991 Jun;173(11):3419-24. doi: 10.1128/jb.173.11.3419-3424.1991. J Bacteriol. 1991. PMID: 1710617 Free PMC article.
-
Gene- and strand-specific repair in vitro: partial purification of a transcription-repair coupling factor.Proc Natl Acad Sci U S A. 1991 Sep 15;88(18):8232-6. doi: 10.1073/pnas.88.18.8232. Proc Natl Acad Sci U S A. 1991. PMID: 1896474 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
