Isolation of adenosine 5'-diphosphate-L-glycero-D-mannoheptose, the assumed substrate of heptose transferase(s), from Salmonella minnesota R595 and Shigella sonnei Re mutants

J Biol Chem. 1984 Oct 10;259(19):11858-60.

Abstract

From heptose transferase-less Re mutants of Salmonella minnesota and Shigella sonnei, a mixture of nucleotide-linked heptoses was isolated. After paper chromatography in different solvent systems, ADP derivatives of D-glycero-D-mannoheptose and L-glycero-D-mannoheptose could be isolated in pure form. The structure of ADP-L-glycero-D-mannoheptose was verified by analytical methods and by transformation of ADP-D-glycero-D-mannoheptose with ADP-D-glycero-D-mannoheptose-6-epimerase.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Diphosphate Sugars / isolation & purification*
  • Chromatography, Gas
  • Chromatography, Ion Exchange
  • Mutation
  • Nucleoside Diphosphate Sugars / isolation & purification*
  • Salmonella / enzymology*
  • Salmonella / genetics
  • Shigella sonnei / enzymology*
  • Shigella sonnei / genetics
  • Transferases / genetics
  • Transferases / metabolism*

Substances

  • Adenosine Diphosphate Sugars
  • Nucleoside Diphosphate Sugars
  • adenosine 5'-diphosphate-glycero-mannoheptose
  • Transferases