Abstract
From heptose transferase-less Re mutants of Salmonella minnesota and Shigella sonnei, a mixture of nucleotide-linked heptoses was isolated. After paper chromatography in different solvent systems, ADP derivatives of D-glycero-D-mannoheptose and L-glycero-D-mannoheptose could be isolated in pure form. The structure of ADP-L-glycero-D-mannoheptose was verified by analytical methods and by transformation of ADP-D-glycero-D-mannoheptose with ADP-D-glycero-D-mannoheptose-6-epimerase.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adenosine Diphosphate Sugars / isolation & purification*
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Chromatography, Gas
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Chromatography, Ion Exchange
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Mutation
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Nucleoside Diphosphate Sugars / isolation & purification*
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Salmonella / enzymology*
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Salmonella / genetics
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Shigella sonnei / enzymology*
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Shigella sonnei / genetics
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Transferases / genetics
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Transferases / metabolism*
Substances
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Adenosine Diphosphate Sugars
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Nucleoside Diphosphate Sugars
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adenosine 5'-diphosphate-glycero-mannoheptose
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Transferases