Production and release of toxins A and B by Clostridium difficile

J Med Microbiol. 1984 Dec;18(3):385-91. doi: 10.1099/00222615-18-3-385.

Abstract

The production and release of toxins A and B by Clostridium difficile during in-vitro culture was investigated. Cell-associated toxin A was detected by immunoelectrophoresis of bacterial extracts released by ultrasonication and by fluorescent antibody labelling of whole cells. Extracellular toxin A was detected by immunoelectrophoresis and by enzyme-linked immunosorbent assay; extracellular toxin B was detected by cytotoxin assay. Both toxins A and B were produced and released during the decline phase of the bacterial growth cycle. The possible significance of these results in relation to the pathogenesis of pseudomembranous colitis is discussed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Proteins*
  • Bacterial Toxins / analysis
  • Bacterial Toxins / biosynthesis*
  • Bacterial Toxins / metabolism
  • Clostridium / growth & development
  • Clostridium / metabolism*
  • Cytotoxins / analysis
  • Cytotoxins / biosynthesis*
  • Cytotoxins / metabolism
  • Enzyme-Linked Immunosorbent Assay
  • Fluorescent Antibody Technique
  • Immunoelectrophoresis, Two-Dimensional

Substances

  • Bacterial Proteins
  • Bacterial Toxins
  • Cytotoxins
  • toxB protein, Clostridium difficile